Physiological and interactomic analysis reveals versatile functions of Arabidopsis 14-3-3 quadruple mutants in response to Fe deficiency

Abstract To date, few phenotypes have been described for Arabidopsis 14-3-3 mutants or the showing role of in plant responding to abiotic stress. Although one member protein family (14-3-3 omicron ) was shown be involved proper operation Fe acquisition mechanisms at physiological and gene expression levels thaliana , it remains explored whether other members play a regulating iron acquisition. more directly effectively observe non-epsilon group function Fe-deficiency adaptation, three higher order quadruple KOs, kappa/lambda/phi/chi ( klpc ), kappa/lambda/upsilon/nu( klu n), upsilon/nu/phi/chi unpc were generated studied analysis this study. The iron-utilization efficiency, root phenotyping, transcriptional level Fe-responsive genes suggested that mutant with kl background showed different from Wt when plants suffered starved, while these absent mutant. Moreover, absence four isoforms klun has clear impact on interactome upon deficiency. Dynamics 14-3-3-client interactions 27 17 proteins differentially interacted roots caused by deficiency, respectively. Many responsive glycolysis, oxidative phosphorylation TCA cycle, FoF1-synthase cysteine/methionine synthesis. A explanation observed awaits detailed functional aspects binding target identified